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LNA-2'OMeRNA/RNA Calculator

Prediction of thermodynamic parameters (free energy, enthalpy, entropy, and melting temperature) for LNA-2’OMeRNA/RNA duplexes:


LNA-2’OMeRNA strand sequence
[NaCl] M
Limit: 0.01 – 1 M
Duplex:

Results:



Important:
1/ use A, C, G and U for 2’-O-methylnucleotides and D for nucleotide of 2,6-diaminopurine-2’-O-methylriboside whereas a, c, g, u and d for respective LNA nucleotides. Do not use space, tabs and other characters,

2/ LNA-2’OMeRNA means 2’-O-methyl oligoribonucleotide containing LNA at selected positions whereas RNA means oligoribonucleotide,

3/ Thermodynamic parameters are calculated for 10-4M oligomer concentration,

4/ RNA strand is added automatically,

5/ References.