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Thermodynamic calculators allow to calculate the thermodynamic parameters such as enthalpy (∆Ho), entropy(∆So), free energy (∆Go37) and melting temperature (Tm) of two types of the duplexes. 2’-OMeRNA/RNA calculator is for duplexes formed by 2’-O-methylRNA and RNA. LNA-2’-OMeRNA/RNA calculator is for duplexes formed by 2’-O-methylRNA containing LNA nucleotides at selected position and RNA. More information about thermodynamic parameters used for calculation of those duplexes can be found in the following publications:
| 1 |
E. Kierzek, A. Ciesielska, K. Pasternak, D.H. Mathews, D.H. Turner and R. Kierzek,
The influence of locked nucleic acid residues on thermodynamic properties of
2’-O-methyl RNA/RNA hetroduplexes.
Nucleic Acids Research, 33 (16), 5082-5093 (2005) |
| 2 |
E. Kierzek, D.H. Mathews, A. Ciesielska, D.H. Turner and R. Kierzek,
Nearest neighbor parameters for Watson-Crick complementary heteroduplex
formed between 2’-O-methyl RNA and RNA oligonucleotides.
Nucleic Acids Research, 34 (13), 3609-3614 (2006) |
| 3 |
A. Pasternak, E. Kierzek, K. Pasternak, D. H. Turner and R. Kierzek,
A chemical synthesis of LNA-2,6-diaminopurine riboside, and the influence of
2’-O-methyl-2,6-diaminopurine and LNA-2,6-diaminopurine ribosides on the
thermodynamic properties of 2’-O-methyl RNA/RNA heteroduplexes.
Nucleic Acids Research, 35 (12), 4055-4063 (2007) |
| 4 |
E. Kierzek, R. Kierzek, W. Moss, S. Christensen, T. Eickbush, D.H. Turner,
Isoenergetic Penta- and Hexanucleotide Microarray Probing and Chemical Mapping Provide a Secondary Structure Model for an RNA Element Orchestrating R2 Retrotransposon Protein Function.
Nucleic Acids Research, 36, 1770-1782 (2008) |
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