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Dr. Ewa Biała

biala[at]ibch.poznan.pl

Current research activity of Dr Ewa Biała

Dr. Ewa Biala is currently working on the application of antisense oligonucleotides to promote allele specific degradation of mRNA containing trinucleotide CAG repeats. Such CAG repeats are correlated with Machado-Joseph's disease. In in vitro experiments she has observed significant increase in the selectivity of pathogenic mRNA degradation. Presently, she is working to optimize in vivo experiments using HeLa cells.

Education
PhD degree (1977) Chemistry, Adam Mickiewicz University, Department of Chemistry, Poznań, Poland
MS degree (1970) Chemistry, Adam Mickiewicz University, Department of Chemistry, Poznań, Poland
International training (total of 43 months)

  • Research fellow, Institute of Organic Chemistry, University of Basel, Dr P. Strazewski, Switzerland, 23 months

  • Research fellow, University Chemical Laboratories, Cambridge, Dr O. Kennard and Laboratory of Molecular Biology, Dr M.T. Gait, 9 months

  • Research fellow, Department of Chemistry, University of Birmingham, Prof. A.S. Jones, 11 months


    • Scientific Awards of Dr Ewa Biala

  • Award from the president of Polish Academy of Sciences, 1977

  • Award from the president of Polish Academy of Sciences, 1987


    • Full list of publications, total citations: 523, Hirsch index: 11
    1 W.Z. Antkowiak, E. Biala, P. Dembek, R. Kierzek, K. Grzeskowiak, A. Kraszewski, M. Wiewiorowski, Further studies on synthesis and properties of the protected triester nucleotides as a new key intermediate for the synthesis of oligoribonucleotides. Nucleic Acids Res., spec. publ. No 1, 133-136 (1975)
    2 R.W. Adamiak, M.Z. Barciszewska, E. Biala, K. Grzeskowiak, R. Kierzek, A. Kraszewski, W. T. Markiewicz, M. Wiewiorowski, Nucleoside 3'-phosphotriesters as key intermediates for the oligonucleotide synthesis. An improved preparation of nucleoside 3'-phosphotriesters, their 1H NMR characterization and new condition for removal of 2-cyanoethyl group. Nucleic Acids Res., 3, 3397-3408 (1976)
    3 R.W. Adamiak, E. Biala, K. Grzeskowiak, R. Kierzek, A. Kraszewski, W.T. Markiewicz, J. Stawinski, M. Wiewiorowski, Nucleoside 3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. IV. New method for removal of 2,2,2-trichloroethyl group and 31P NMR as a new tool for analysis of deblocking of internucleotide phosphate protecting groups. Nucleic Acids Res., 4, 2321-2329 (1977)
    4 R.W. Adamiak, E. Biala, K. Grzeskowiak, R. Kierzek, A. Kraszewski, W.T. Markiewicz, J. Okupniak, J. Stawinski, M. Wiewiorowski, The chemical synthesis of the anticodon loop of an eukaryotic initiator tRNA containing the hypermodified nucleoside N-(N-threonylcarbonyl)adenosine. Nucleic Acids Res., 5, 1889-1905 (1978)
    5 R.W. Adamiak, E. Biala, K. Grzeskowiak, R. Kierzek, A. Kraszewski, W.T. Markiewicz, J. Stawinski, M. Wiewiorowski, Studies on polynucleotide synthesis. in: Phosphorus Chemistry directed towards Biology, ed. W.J.Stec, Pergamon Press, Oxford New York, 9-12 (1980)
    6 E. Biala, A.S. Jones, R.T. Walker, Synthesis and properties of poly(5-ethynyluridylic acid). Tetrahedron, 36,155-164 (1980)
    7 W.T. Markiewicz, R.W. Adamiak, E. Biala, K.Grzeskowiak, R. Kierzek, A.Kraszewski, J.Stawinski, M.Wiewiorowski, Further studies on oligoribonucleotide synthesis. Nucleic Acids Res., symp. ser., 7, 115-127 (1980)
    8 W.T. Markiewicz, E. Biala, R. Kierzek, Application of the tetraisopropyldisiloxane-1,3-diyl group in the chemical synthesis of oligoribonucleotides. Bull. Acad. Polon. Sci., ser. chim., 32, 433-451 (1984)
    9 R.W. Adamiak, E. Biala, Scale-up procedure for the total deprotection and purification of the chemically synthesized oligoribonucleotides. Nucleic Acids Res. Symp.Ser., 14, 305-309 (1984)
    10 R.W. Adamiak, E. Biala, B. Skalski, New ionic side-products in oligonucleotide synthesis: formation and reactivity of fluorescent N-(purin-6-yl) pyridinium salts. Nucleic Acids Res., 13, 2989-3003 (1985)
    11 R.W. Adamiak, E. Biala, B. Skalski, Synthesis of 6-substituted purines and ribonucleosides with N-(6-purinyl) pyridinium Salts. Angew. Chem. Int. Ed. Engl., 24, 1054-1055 (1985)
    12 R.W. Adamiak, E. Biala, Z. Gdaniec, S. Mielewczyk, B. Skalski, Reactions of nucleoside heteroaromatic lactam systems with 4-chlorophenylphosphorodichloridate and 1,2,4-triazole in pyridine. Chemica Scripta 26, 3-6 (1986)
    13 R.W. Adamiak, E. Biala, Z. Gdaniec, S. Mielewczyk, B. Skalski, New observation on nucleophile assisted phosphorylation of nucleoside heteroaromatic lactam sysytem during oligonucleotide synthesis. Formation and reactivity of ionic side products. Chemica Scripta, 26, 7-10 (1986)
    14 R.W. Adamiak, E. Biala, Z. Gdaniec, S. Mielewczyk, B. Skalski, Studies directed toward introduction of N6-isopenthenyladenosine and its 2-methylthio-analogs into oligoribonucleotides. Nucleic Acids Res. Symp. Ser.,18, 105-109 (1987)
    15 G. Slim, C. Pritchard, E. Biala, U. Asseline, M. Gait, Synthesis of site-specifically modified ologoribonucleotides for studies of the recognition of TAR RNA by HIV-1 tat protein and studies on hammerhead ribozymes. Nucleic Acids Res. Symp. Ser., 24, 55-59 (1991)
    16 E. Biala, J. Milecki, A. Kowalewski, M. Popenda, W.Z. Antkowiak, Z-RNA. The synthesis of 5-methyl and 2`-O-methyl analogs of ribo-CGCGCG. Acta Biochim. Pol., 40, 521-529 (1993)
    17 W.B.T. Cruse, P. Saludjian, E. Biala P. Strazewski, T. Prange, O. Kennard, Structure of a mispaired RNA double helix at 1.6 A resolution and implication for the prediction of RNA secondary structure. Proc. Natl. Acad. Sci. USA., 91, 4160-4165 (1994)
    18 M. Popenda, J. Milecki, E. Biala, R.W. Adamiak, The solutions of NMR structure of 2`-O-(13C)methyl-CGCGCG RNA duplex. Nucleosides & Nucleotides, 14, 983-988 (1995)
    19 A. Burdzy, B. Skalski, E. Biala, A. Kowalewski, S. Paszyc, R.W. Adamiak, Post-synthetic transformations of oligodeoxynucleotides originated at 6-methylthio-purine site. Nucleosides & Nucleotides, 14, 978-982 (1995)
    20 A. Fischer, Z. Gdaniec, E. Biala, M. Lozynski, J. Milecki, R.W. Adamiak, 19F NMR of RNA. The structural and chemical aspects of 5-fluorocytidine and -uridine labelling of oligoribonucleotides. Nucleosides & Nucleotides 15, 477-488 (1996)
    21 M. Popenda, E. Biala, J. Milecki, R.W. Adamiak, Solution structure of RNA duplexes containing alternating CG base pairs: NMR study of r(CGCGCG)2 and 2`-O-Me(CGCGCG)2 under low salt conditions. Nucleic Acids Res., 25, 4589-4598 (1997)
    22 S. Gunzenhauser, E. Biala, P. Strazewski, Tetraethylene glycol-derived spacer for oligonucleotide synthesis. Tetrahedron Lett., 39, 6277-6280 (1998)
    23 P. Strazewski, E. Biala, K.Gabriel, W.H. McClain, The relationship of thermodynamic stability at a GU recognition site to tRNA aminoacylation specifity. RNA, 5, 1490-1494 (1999)
    24 E. Biala, P.Strazewski, Thermodynamics of site-specific variant tRNAAla acceptor stem microharpins. Nucleosides & Nucleotides, 18, 1575-1576 (1999)
    25 E. Biala, O. Botta, E. Moyroud, P.Strazewski, Ribonucleosides, aminoacyl- and peptidyl-RNA: synthesis, thermodynamics and structure. Chimia, 53, 226-227 (1999)
    26 E. Moyroud, E. Biala, P. Strazewski, Synthesis and enzymatic digestion of an RNA nonamer in both enantiomeric forms. Tetrahedron, 56, 1475-1484 (2000)
    27 E. Kierzek, E. Biala and R. Kierzek, Elements of thermodynamics in RNA evolution. Acta Biochemica Polonica, 48, 485-493 (2001)
    28 K. Ziomek, E. Kierzek, E. Biala and R. Kierzek, The thermal stability of RNA duplexes containing modified base pairs placed at internal and terminal positions of the oligoribonucleotides. Biophysical Chemistry, 97, 233-241 (2002)
    29 K. Ziomek, E. Kierzek, E. Biala and R. Kierzek, The influence of various modified nucleotides placed as 3'-dangling end on thermal stability of RNA duplexes. Biophysical Chemistry, 97, 243-249 (2002)
    30 S. Terenzi S. E. Biala E. N,Q. Nguyen-Trung and P. Strazewski, Amphiphilic 3'-peptydyl-RNA conjugates Angewandte Chemie Internat. Edition, 42, 2909-2912 (2003)